optical fluorescence microscope longpass bandpass filter set
optical fluorescence microscope longpass bandpass filter set

Fluorescent Longpass TRITC Microscope Filter

Fluorescent Longpass TRITC Filter Microscope Filter Set manufactured by Coligh is engineered for detection of TRITC and other orange-emitting fluorophores.

It has key features below:

  • Excitation Filter: 530/30 nm
  • Emission Filter: LP575 (Longpass)
  • Dichroic Mirror: 565 nm
  • High transmission and deep blocking depth
  • Hard Coated Technology
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Longpass Microscope Filter for Orange TRITC Fluorescence Detection Description

Coligh manufactures fluorescent longpass TRITC microscope filter set specifically optimized for TRITC fluorescent dyes, ideal for orange-red fluorescence detection in multicolor imaging systems.

  • Excitation filter 530/30 nm: Precisely matches the TRITC absorption peak (550 nm), with >90% transmission to deliver more effective photons.

  • Emission filter LP575 nm: Fully captures the extended TRITC emission spectrum (580–650 nm), avoiding signal cutoff seen with bandpass filters.

  • Dichroic mirror 565 nm: Effectively reflects excitation light and transmits emission light, achieving precise separation at 565 nm and directing the appropriate light paths to the sample and detector.

  • Blocking depth >OD5: Strong suppression of out-of-band light and non-specific excitation improves fluorescence image contrast.

  • >90% transmission: Enables stronger fluorescence signals and improves detection of weak emissions.

  • Hard-coated by dry deposition: Scratch-resistant and durable, suitable for long-term integration in industrial systems or frequent module replacement in lab environments.

Fluorescent Longpass TRITC Microscope Filter Technical Datasheet

Item Specification
Product Model Fluorescent Longpass TRITC Microscope Filter
Application TRITC
Excitation Filter 530/30 nm (CWL 530nm, FWHM 30 nm), OD >5, Tp> 95%
Allowance for center wavelength and FWHM: +/-2nm
Blocking depth: 200-1100nm@OD>5
Emission Filter Longpass 575 nm, OD > 5 @250-550nm
Allowance for cut on wavelength: +/-2nm
Dichroic Mirror Cut-on at 565 nm, AOI 45°, R > 95% @ 500-550nm, T > 90% @570-750nm
Filter Sizes (EX & EM) Ø25.0 mm × 3.5 mm mounted
Dichroic Mirror Size 36.0 mm × 26.0 mm × 1.0 mm
Angle of Incidence 0° (EX/EM), 45° (Dichroic)
Substrate Material Fused Silica
Coating Type Ion-assisted deposition + sputtered hard coating
Surface Quality 80/50 60/40 (per MIL-PRF-13830B)
Compatible Microscopes Nikon, Olympus, Zeiss, Leica

Fluorescent Longpass TRITC Microscope Filter Applications

  • Cell Fluorescence Imaging

In cell research, TRITC is commonly used to label α-tubulin, actin, or cytoskeletal proteins. Due to the fine structure of organelles, high resolution and signal-to-noise ratio are critical. Traditional filters with narrow bandwidths may cut off the tail end of TRITC’s emission spectrum, resulting in signal loss; while wideband filters with insufficient blocking may introduce background noise. This filter set, with its LP575 longpass design, fully captures TRITC’s 580–650 nm emission range and avoids losing critical signal.

  • Multicolor Fluorescence Imaging

In tissue sections or cells, it is common to label multiple targets—for example, DAPI for nuclei, FITC for surface proteins, and TRITC for intracellular proteins. Each fluorophore requires precise excitation/emission separation to avoid signal crosstalk or spectral overlap. TRITC’s emission overlaps with Cy3 and mCherry in the red-orange region, so accurate filter separation is essential. This filter set can be used alongside others to effectively reduce signal interference and enable simultaneous multi-target detection.

  • Live-cell Imaging

Live-cell experiments present two major challenges: strong excitation without causing phototoxicity, and optical components that remain stable under varying temperature and humidity. TRITC has relatively weak emission in live-cell conditions, making high transmission, low background, and durable filters essential. This filter set uses hard-coated technology to ensure long-term stability, resistance to delamination, and compatibility with CO₂ incubators and humid environments.

 

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