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545nm 678nm Multiband Dichroic Mirror Beam Splitter
545nm 678nm Multiband Dichroic Mirror Beam Splitter is a multi-band dichroic mirror designed to reflect 545nm and 678nm at a 45° and transmit light in other bands. Coligh manufactures dichroic mirrors.
It has features below:
- Dual band reflection
- Broad band transmission
- 45 deg AOI
- Hard coating
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Dual-Band Dichroic Mirror 545nm 678nm Hard Coated Description
545nm/678nm Multiband Dichroic Mirror Beam Splitter is specifically designed to provide dual-channel separation in multicolor fluorescence systems.
- It offers dual reflection bands: >95% reflectance in the 525–565 nm and 669–687 nm ranges, enabling efficient detection of green signals such as FITC/GFP and deep red signals like Cy5/chlorophyll-a.
- Dual transmission bands allow ≥93% transmission in the 590–640 nm and 720–800 nm ranges, suitable for transmitting NIR fluorescence signals such as RFP and Cy7.
- Optimized for systems using a 45° AOI, making it ideal for standard fluorescence microscopes, flow cytometers, imaging spectrometers, and optical sensing setups.
- We use a hard coating multilayer structure for the dichroic mirror to ensure long operational life.
545nm 678nm Multiband Dichroic Mirror Beam Splitter Technical Datasheet
| Parameter | Specification |
| Reflection Bands | 525–565 nm, 669–687 nm |
| Reflectance in Reflection Bands | > 95% |
| Transmission Bands | 590–640 nm, 720–800 nm |
| Transmission in Transmission Bands | ≥ 93% |
| Angle of Incidence (AOI) | 45° ± 3.6° |
| Coating Type | Hard-coated dielectric multilayer |
| Substrate Material | Optical glass or fused silica |
| Surface Quality | 60/40 (per MIL-PRF-13830B) |
| Flatness | λ/4 |
| Dimensions | Custom sizes available |
| Thickness | 1.0 mm ± 0.1 mm (customizable) |
| Clear Aperture | > 90% of central area |
545nm 678nm Multiband Dichroic Mirror Beam Splitter Applications
- Multicolor Fluorescence Microscopy
In experiments requiring simultaneous observation of multiple cellular structures or molecules labeled with fluorophores of different wavelengths, it is essential to efficiently separate and direct the emission signals to different detectors. The 545nm 678nm Multiband Dichroic Mirror Beam Splitter can be used in combination with excitation and emission filters to further separate the two channels, enabling simultaneous signal acquisition without filter wheel switching. This is particularly beneficial for live-cell dynamic imaging. - Flow Cytometry
When analyzing complex cell populations, multiple parameters labeled with fluorophores of different emission spectra need to be detected simultaneously. The 545nm 678nm Multiband Dichroic Mirror Beam Splitter efficiently reflects target wavelengths, minimizes signal loss, and precisely separates channels to reduce crosstalk. - FRET Imaging
FRET experiments require precise separation of donor emission, acceptor emission, and potential FRET signals. Sometimes, it is necessary to monitor both donor and acceptor simultaneously. This dual-band dichroic mirror can accurately separate the acceptor and FRET emission while efficiently reflecting key wavelengths, and allowing donor signal transmission, enabling high-efficiency, simultaneous acquisition of donor and acceptor/FRET signals.
