488nm Interference Filter FWHM 12nm
488nm Interference Filter FWHM 12nm is designed to transmit light centered 488nm and FWHM 12nm. Coligh manufactures interference bandpass filter from EUV to IR.
- Center wavelength 488
- FWHM 12nm
- Transmission peak ≥90%
- Blocking depth OD6
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Narrowband 488nm Interference Filter FWHM 12nm Description
488nm Interference Filter FWHM 12nm Coligh manufactures Central wavelength 488+/-1nm, half bandwidth 12+/-1nm, specially designed for blue light band, suitable for exciting or detecting specific fluorescent markers such as FITC, GFP, etc.
- Half bandwidth 12nm can ensure high wavelength selectivity and excellent spectral resolution, effectively suppressing background stray light interference.
- Peak transmittance is greater than or equal to 90%, retaining the target signal intensity to the greatest extent and improving detection sensitivity.
- Cut-off depth is greater than or equal to OD6 in the range of 350-1000nm, achieving efficient light blocking in the non-passband wavelength range and reducing noise.
- We use hard-coated multi-layer dielectric interference coating technology, the film layer is strong and durable, resistant to moisture and heat, and resistant to wipe, suitable for long-term use. And with the base fused quartz, it can meet the mechanical strength and thermal stability requirements of different scenarios.
488nm Interference Filter FWHM 12nm Technical Datasheet
Parameter | Specification |
Center Wavelength (CWL) | 488 nm ± 1 nm |
Full Width at Half Maximum (FWHM) | 12nm± 1 nm |
Peak Transmission | 90% |
Blocking Range | 350-1000nm |
Optical Density (OD) | OD ≥ 6 outside passband |
Angle of Incidence (AOI) | 0° +/-5° |
Surface Quality | 60/40 scratch-dig (per MIL-PRF-13830B) |
Material Substrate | Fused Silica JGS1 |
Coating Type | Hard-Coated Interference Filter/ IAD Assisted Coating |
Thickness | 1mm (typical) |
Filter Dimensions | Custom sizes available (e.g., Ø12.5 mm, 60×60 mm) |
Clear Aperture | >90% of physical size |
Operating Temperature | -40°C to +85°C |
488nm Interference Filter FWHM 12nm Applications
- Flow cytometer
During cell sorting and detection, flow cytometers need to accurately excite and detect fluorescent markers such as FITC and GFP, but stray light such as lasers or ambient light will interfere with cell signal recognition. The 488/12nm narrowband filter can be integrated into the excitation light path or detection module and used in conjunction with the 488nm laser to filter light of non-target wavelengths. By allowing only narrowband light of 488nm±6nm to pass through, the excitation/emission spectra of fluorescent dyes are accurately matched. : Reduce false detection and ensure cell sorting accuracy - Fluorescence microscope
In fluorescence imaging, background noise from autofluorescence or nonspecific signals will reduce the imaging contrast of GFP protein. The 488/12nm optical filter can be installed on the excitation light source or camera detection end of the microscope as an excitation filter or emission filter to ensure that only the target fluorophore is excited. Or block the reflected excitation light and allow only the fluorescent signal to enter the camera. Make weak fluorescent signals clearly visible and support super-resolution imaging. - Industrial laser processing
In laser processing equipment, the sensor may be disturbed by reflected light or other light sources in the processing area, resulting in distortion of the control signal. The 488/12nm interference filter can be integrated at the output end of the laser or the receiving end of the sensor as a protective filter component. By only allowing the 488nm working laser to pass through, other wavelengths of stray light are blocked to avoid false triggering of the laser control signal and ensure cutting/engraving consistency. - Raman spectrometer
The Raman signal is weak and easily submerged by the Rayleigh scattering of the excitation light, so the background noise needs to be filtered out efficiently. The 488/12nm narrowband filter can be used as a pre-filter of the laser or a bandpass filter at the spectral detection end. Purify the 488nm laser to ensure the monochromaticity of the light source. Only collect Raman scattering signals and suppress Rayleigh scattering light. Accurately identify the characteristic peaks of the material (